AAV purification protocol using chromatorgraphy columns for serotype 8?

01 January 2018 1 6K Report

Does anyone have a protocol (that has worked) or can suggest a protocol of AAV8 purification using an ion exchange column or any type of chromatography. I am looking for a procedure that does not require unltracentrifugation. Thanks!

Simon N Waddington

Dear Deemah,

Please email me (s.waddington[at]ucl.ac.uk) and I can send you a comprehensive protocol.

BW, Simon

Badges
Science topic

Similar topics
Analytical Chemistry
Column

More Deemah Dabbagh's questions See All

Purification of AAV by ion exchange chromatography. How to adjust pH of virus lysate?

I would like to purify AAV particles using ion exchange chromatography. I am testing different pH levels for elution. Is it ok (safe for the virus particles) to use HCL or NaoH to...

08 September 2018 9,202 1 View

Density gradient- and serotype-independent method of AAV purification?

Dear all, Has anyone tried purifying recombinant AAV virus without density gradient ultracentrifugation in a serotype indepedent manner. Also, Is there a way to achieve high titer without...

11 December 2017 591 4 View

Does any one know if there are anti-HBV antibodies for western blot that recognize multiple HBV antigens?

It would be helpful if the antibody can be used for ELISA and Flow cytometry also.

02 March 2016 1,696 2 View

Virus particle purification?

I am transfecting T293 cells with pAAV (adeno-associated virus vector). How can I purify the recombinant virus after transfection?

01 February 2016 446 4 View

What is the best/cost-effective/fastest method for determining the titer of AAV?

So, I have transfected 293HEK cells with AAV-GFP vector and after purification, I ended up with 50ul of purified virus (or so I hope). What would be the best method to determine my titer with the...

01 February 2016 5,083 5 View

Can someone help with the tubular structures formed by bunyaviruses during their maturation?

Are these structures part of the Golgi membrane itself? or are they independent viral structures?

08 September 2015 1,463 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View