Purification of AAV by ion exchange chromatography. How to adjust pH of virus lysate?

I would like to purify AAV particles using ion exchange chromatography. I am testing different pH levels for elution. Is it ok (safe for the virus particles) to use HCL or NaoH to...

08 September 2018 9,089 1 View

Density gradient- and serotype-independent method of AAV purification?

Dear all, Has anyone tried purifying recombinant AAV virus without density gradient ultracentrifugation in a serotype indepedent manner. Also, Is there a way to achieve high titer without...

11 December 2017 505 4 View

Does any one know if there are anti-HBV antibodies for western blot that recognize multiple HBV antigens?

It would be helpful if the antibody can be used for ELISA and Flow cytometry also.

02 March 2016 1,622 2 View

Virus particle purification?

I am transfecting T293 cells with pAAV (adeno-associated virus vector). How can I purify the recombinant virus after transfection?

01 February 2016 368 4 View

What is the best/cost-effective/fastest method for determining the titer of AAV?

So, I have transfected 293HEK cells with AAV-GFP vector and after purification, I ended up with 50ul of purified virus (or so I hope). What would be the best method to determine my titer with the...

01 February 2016 5,015 5 View

Can someone help with the tubular structures formed by bunyaviruses during their maturation?

Are these structures part of the Golgi membrane itself? or are they independent viral structures?

08 September 2015 1,308 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View