Deemah Dabbagh

7 Questions 7 Answers 0 Followers

Questions related from Deemah Dabbagh

Purification of AAV by ion exchange chromatography. How to adjust pH of virus lysate?

I would like to purify AAV particles using ion exchange chromatography. I am testing different pH levels for elution. Is it ok (safe for the virus particles) to use HCL or NaoH to...

09 September 2018 9,169 1 View

AAV purification protocol using chromatorgraphy columns for serotype 8?

Does anyone have a protocol (that has worked) or can suggest a protocol of AAV8 purification using an ion exchange column or any type of chromatography. I am looking for a procedure that does not...

01 January 2018 6,176 1 View

Density gradient- and serotype-independent method of AAV purification?

Dear all, Has anyone tried purifying recombinant AAV virus without density gradient ultracentrifugation in a serotype indepedent manner. Also, Is there a way to achieve high titer without...

12 December 2017 561 4 View

Does any one know if there are anti-HBV antibodies for western blot that recognize multiple HBV antigens?

It would be helpful if the antibody can be used for ELISA and Flow cytometry also.

03 March 2016 1,672 2 View

Virus particle purification?

I am transfecting T293 cells with pAAV (adeno-associated virus vector). How can I purify the recombinant virus after transfection?

02 February 2016 420 4 View

What is the best/cost-effective/fastest method for determining the titer of AAV?

So, I have transfected 293HEK cells with AAV-GFP vector and after purification, I ended up with 50ul of purified virus (or so I hope). What would be the best method to determine my titer with the...

02 February 2016 5,060 5 View

Can someone help with the tubular structures formed by bunyaviruses during their maturation?

Are these structures part of the Golgi membrane itself? or are they independent viral structures?

09 September 2015 1,430 1 View