Does any body have experience regarding the transfection of BMI-1 gene into MSCs (Ad-MSCs) through lipofectamine?

04 April 2018 0 2K Report

i need recommendations/advice regarding the transfection of BMI-1 gene in to Adipose derived stem cells through lipofectamine. We are transfecting the BMI1 gene through lipofectamine, the cells show good GFP expression but they cant proliferate and end in apoptosis. how is it possible to establish BMI-1 expressing Ad-MSCs (cell) line?

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Science method

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