10 Questions 15 Answers 0 Followers
Questions related from Imdad Ullah Khan
i need recommendations/advice regarding the transfection of BMI-1 gene in to Adipose derived stem cells through lipofectamine. We are transfecting the BMI1 gene through lipofectamine, the cells...
04 April 2018 1,810 0 View
what is the best way to express the western blot data. mostly it is expressed as relative to sample control, but i also found some papers where the data was expressed as Realtive to beta actin. i...
03 March 2018 1,119 4 View
I need some guidance; as asked by the reviewer to put q value of ANOVA post hoc-tukey with a p-value, how i will write the q value in the manuscript to describe multiple group comparisons, and is...
12 December 2017 9,325 1 View
can i get Q or F value with post hoc tukeys or man whiteny U test on SPSS? as i am a beginner to statistics i do not have a sufficient knowledge whether is it possible; however it can be...
11 November 2017 8,442 3 View
i need to know the pathway which allow the hemeoxygenase-1 to induce the BDNF production in brain injuries.
09 September 2017 9,037 0 View
i am using stripping buffer for 1hr to remove the previous bands but still the previous band are visible. is it possible to completely remove the previous bands with stripping buffer ? .
08 August 2016 716 4 View
I am getting multiple bands for TNF-alpha and IL-6 on western blot membrane which make me difficult to search for the desire band. is it due to problem with the primary antibody or improper washing?
08 August 2016 3,281 10 View
after protein transfer how long the membrane can be stored in TBST ? Is the protein quality deteriorated if the membrane is stored for a prolong period of time?
08 August 2016 1,328 5 View
how long the protein sample can be store for western blot analysis, does the time length deteriorate the quality of proteins to be tested?
06 June 2016 255 7 View
i am doing western blot for my research study and i am getting the black spots on the membrane after developing which indicate the binding of first anti-body to skim milk. how can i eliminate this...
05 May 2016 6,798 9 View
