i need to know the pathway which allow the hemeoxygenase-1 to induce the BDNF production in brain injuries.
i need recommendations/advice regarding the transfection of BMI-1 gene in to Adipose derived stem cells through lipofectamine. We are transfecting the BMI1 gene through lipofectamine, the cells...
03 April 2018 1,751 0 View
what is the best way to express the western blot data. mostly it is expressed as relative to sample control, but i also found some papers where the data was expressed as Realtive to beta actin. i...
02 March 2018 1,052 4 View
I need some guidance; as asked by the reviewer to put q value of ANOVA post hoc-tukey with a p-value, how i will write the q value in the manuscript to describe multiple group comparisons, and is...
11 December 2017 9,259 1 View
can i get Q or F value with post hoc tukeys or man whiteny U test on SPSS? as i am a beginner to statistics i do not have a sufficient knowledge whether is it possible; however it can be...
10 November 2017 8,354 3 View
I am getting multiple bands for TNF-alpha and IL-6 on western blot membrane which make me difficult to search for the desire band. is it due to problem with the primary antibody or improper washing?
07 August 2016 3,216 10 View
after protein transfer how long the membrane can be stored in TBST ? Is the protein quality deteriorated if the membrane is stored for a prolong period of time?
07 August 2016 1,252 5 View
i am using stripping buffer for 1hr to remove the previous bands but still the previous band are visible. is it possible to completely remove the previous bands with stripping buffer ? .
07 August 2016 643 4 View
how long the protein sample can be store for western blot analysis, does the time length deteriorate the quality of proteins to be tested?
05 June 2016 188 7 View
i am doing western blot for my research study and i am getting the black spots on the membrane after developing which indicate the binding of first anti-body to skim milk. how can i eliminate this...
04 May 2016 6,698 9 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?
02 March 2021 4,565 3 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
Source to get Impact factor of journals
01 March 2021 6,654 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...
26 February 2021 928 6 View
There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.
26 February 2021 5,851 1 View
I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...
25 February 2021 6,635 5 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View