after protein transfer how long the membrane can be stored in TBST ? Is the protein quality deteriorated if the membrane is stored for a prolong period of time?
Hello Imdad
in short I woouls store the membrane dry inside a cassette or dessicated environment
If you leave your membrane in TBST for more than 24 hors even @ 4C proteins can begin to come away
ooh,
thank you Mr Hall.
can you plz specify more about the cassette, it is a special cassette or i can be stored any where after drying ?
Hello Imdad
I was referring to a standard metal caste that you use for blots
However, if you image your membrane directly, then wrap in cling film/ saran wrap and keep in a cool dark dry place until you use again
When you use the membrane again simply block for another 1 hour @ room temp in PBST/TBST + 1 % BSA or skimmed milk and then proceed as you would first time around, i.e. add primary antibody etc
I rinse the PVDF membrane and dry them in the air. Place it in between two kimwipe sheets, place in the ziplock plastic bag and keep it in a -20C freezer for several years (I believe)
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