What are the contents of the supplication of the oppressed that Imam Al-Hadi (peace be upon him) teaches us?

What are the contents of the supplication of the oppressed that Imam Al-Hadi (peace be upon him) teaches us?

08 February 2021 6,791 5 View

Do you think Writing Process is essential for EFL students to teach?

05 April 2016 9,336 10 View

Is it possible to culture RAW264.7 and THP 1 cells without 10% serum ?

hello, Is it possible to culture RAW264.7 and THP 1 cells without 10% serum ? If that can for how long do they live? Where can I find answers for these kind of questions? Can any one suggest a...

08 October 2014 728 7 View

What could be an unexpected stain on inflammatory cells of stomach, on basal cells of tonsilar crypts and muscle cells of large bowel using Cam 5.2?

I am currently doing a comparative study between Becton Dickinson Cam 5.2 and Ventana Cam 5.2. However I had some unexpected results on my control sections. Some inflammatory cells that lined the...

30 August 2013 955 6 View

Thin film solar cell

Can I model a thin film solar cell starting from multilayer thin film Absorptance. If the answer is yes why , I get 100nm thickness is the best thickness for the active layer in calculating...

01 January 1970 7,143 5 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View