Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

What are the dimensions by which the independent variable (big data) can be measured, Knowing We using the questionnaire instrument?

We have a research project consisting of three variables where big data is the independent variable. Some previous studies showed that Volume, Variety, Velocity and Veracity Are the dimensions...

21 February 2021 8,133 2 View

Is nanotechnology part of materials science? Is it possible to obtain Pico technology and move from hypothesis to application?

Pico-scale This is three orders of magnitude smaller than a nanometer (and thus most nanotechnology) and two orders of magnitude smaller than most chemistry transformations and measurements. Pico...

18 February 2021 4,540 5 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

How can we liquify sputum sample to aim efficient M.tb DNA extraction from sputum sample? Can we extract Mtb DNA from sputum treated with NaOH-NALC?

I am currently facing difficulty in the DNA extraction because of the viscosity of the sputum samples. I am using the Chelex based extraction method which does yield in DNA but it is inefficient...

11 December 2019 3,153 1 View

My drugs have higher CAA values than quercetin. Is this logical or did I do something wrong?

Hello, I performed CAA assay with N-acetylcysteine (both free and nano-formulated drugs), quercetin on SHSY-5Y cell line. However, CAA values for those drugs are higher then the quercetin. Any...

07 August 2019 5,475 1 View

What is the best solution for obviating contamination with less RNA destruction?

we use trazol kit for extrcting RNA from sputum ;although we change our extraction kit we faced low 260/280 ratio of nanodrop.(1.5) according to well-known contamination of sputum...

29 January 2019 735 8 View

What is the best solution for obviating contamination with less RNA destruction?

we use trazol kit for extrcting RNA from sputum ;although we change our extraction kit we faced low 260/280 ratio of nanodrop.(1.5) according to well-known contamination of sputum...

31 December 2018 6,823 9 View

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31 December 2018 8,147 3 View

How to prepare NALC buffer for liquefaction of non-TB target sputum?

Can anyone tell me if NALC-NaOH is suitable for liquefaction of sputum for real-time pcr amplification of non TB pathogenic bacteria and virus. Otherwise how to prepare NALC-NaOH or NALC PBS...

31 December 2018 2,649 0 View

How can I process sputum for ELISA assay?

I want to detect some chemokines in sputum samples from patients with cystic fibrosis; in literature there are protocols that recommend the use of DTT 0.1% to dilute the sample, but won't this...

11 December 2018 9,738 4 View

We want to perform VEGF in sputum samples. Can anyone share methodology for processing and storage of these samples for future testing?

We want exact concentrations and pH of reagents required.

10 November 2018 5,123 0 View

How Mycobacterium Tuberculosis sputum specimen can be transported across borders (by Flight) for Genetic Analysis in another Country?

In low resource countries, it is difficult to carryout genotyping studies wheere facilities are not available. i am wondering anyone could give me ideas how Mycobacterium Tuberculosis sputum...

11 September 2018 6,495 8 View