Ewa Stepien

4 Questions 34 Answers 0 Followers

Questions related from Ewa Stepien

Share your experience about extraction of miRNA from plasma or serum. What is the best protocol, pitfalls and tricks you can discuss in public?

miRNA is proposed to be clinical biomarker in many diseases, as well acute and chronic ones. I think that there is a need to validate extraction protocols to introduce miRNA into molecular...

08 August 2014 9,239 34 View

Heparin neutralization in heparanised whole blood samples from patients?

What is the best method to neutralize non-fractionized heparin and LMW heparin in patient blood for coagulation analysis (thromboelastography). Is heparinase I enzyme recommended? Which dose...

08 August 2013 7,959 19 View

Can someone suggest a quantitative method to asses in cells and cell cultures for advanced glycation end products AGE?

The formation of AGEs occurs from the reactive nature of reducing sugars (i.e. glucose) to undergo nonenzymatic rearrangements with amino groups of proteins and DNA to form irreversible...

08 August 2013 253 2 View

Successful method for separation of nanoparticles from isosmotic media (cell culture media)?

I'm looking for an efficient method to sediment zinc oxide nanoparticles, between 20-50 nm in size, from liquids like cell culture media. Filtering is of course a method of choice, but in higher...

05 May 2013 9,436 13 View