Do we need adapt influenza viruses in MDCK cells before conducting serum neutralization test with influenza viruses in MDCK cells ?

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Hicham El Rhaffouli

indeed, it is preferable to adapt the influenza virus to the MDCK cells with 2 or 3 passages before the seroneutralization, especially if the virus has been produced on eggs.

Peter Roberts

Best to adapt to the cells and then lay down a virus stock in the -70 c freezer. Titrate to determine the appropriate dilution to use in the neutralisation assay.

Sivasankar Panickan

Thank you..