Can we use egg adapted influenza virus for serum neutralization test in MDCK cells ?

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Dr. Shahzad Akbar Khan

Yes we can use it.

Thomas Rowe

yes you can. You may have lower neutralization titers than serum obtained from cell-grown virus of the same isolate since there may be egg-adapted changes in the virus due to passage in eggs.

Sivasankar Panickan

Thanks Dr Thomas & Dr Shahzad. actually i am not getting desired titer of 200TCID50 in MDCK cells or i have to infect MDCK monolayer with correct moi. hope eggs also a alternate.

Yes we can use it to infect MDCK monolayer with correct moi