Anybody can give detailed protocol for VNT(virus neutralization) assay for influenza virus?

More Sivasankar Panickan's questions See All

Thresold line value must be same in different runs in time pcr for calculating viral copies?

During standard curve preparation thresold line value is 560. It should be kept same during running test samples for detecting viral rna copies?

15 August 2023 7,730 0 View

How to perform spatial search in higher dimensions efficiently?

I have a 6D grid of approximation order 10. This means, it has a million points: (x, y, z, Vx, Vy, Vz) = (10, 10, 10, 10, 10, 10). Now, I want to find those points of this grid that lie within a...

10 October 2022 2,003 0 View

Can we use egg adapted influenza virus for serum neutralization test in MDCK cells ?

can we use egg adapted influenza virus for serum neutralization test in MDCK cells?Thanks

29 October 2021 2,421 4 View

Do we need adapt influenza viruses in MDCK cells before conducting serum neutralization test with influenza viruses in MDCK cells ?

Do we need adapt influenza viruses in MDCK cells before conducting serum neutralization test with influenza viruses in MDCK cels?? Thanks

29 October 2021 6,004 3 View

Gel purification of linearized plasmid, gave low yield why?

I am using Qiagen Gel extraction kit for gel purification of linearized plasmid. The procedure is followed as per the kit. if i load 1ug DNA in agarose gel, i am getting 600ng DNA or less after...

16 October 2020 6,798 4 View

Dear researchers, In RE digestion with Nco I i am getting smearing. Can any one please advice?

well1- 2 log ladder well2- uncut plasmid(3.2kb) well3- cut wit NcoI well4-cut wit NcoI tried with less time of incubation, low amount of enzyme doesnot work

10 September 2020 6,822 3 View

How to find the list of journals with "Your Paper Your Way"?

Dear Professors and Researchers, Can anyone tell me how to find a list of journals with the choice of "Your Paper Your Way"? Thanks in Advance Regards, Siva

03 May 2020 2,611 5 View

Having a marine actinobacterium. Sequenced for 16S and found to have 98.58% similarity with Citricoccus yambaruensis. Can it be a new species?

Sequenced a marine actinobacterium for 16S, assembled and identified in Eztaxon. Obtained 98.58% similarity with Citricoccus yambaruensis. Can it be a new species? Can I go for whole genome...

09 July 2018 3,412 5 View

Any simple and efficient protocol to explore the diversity of PKS, NRPS and relevant gene in the mangrove sediments?

I would like to explore the PKS, NRPS and relevant gene diversity in the metagenomic actinobacterial DNA of the mangrove sediment samples to understand the secondary metabolite production pattern....

16 November 2017 1,751 1 View

Can we use Soxhlet to extract metabolites from the cell mass of Actinobacteria?

Can we use Soxhlet for extracting the secondary metabolites from the Actinobacteria fermented broth? Soxhlet is generally being used for dry/solid materials. If we adopt this to actinobacteria...

22 October 2017 4,262 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View