Sivasankar Panickan

6 Questions 7 Answers 0 Followers

Questions related from Sivasankar Panickan

Thresold line value must be same in different runs in time pcr for calculating viral copies?

During standard curve preparation thresold line value is 560. It should be kept same during running test samples for detecting viral rna copies?

16 August 2023 7,696 0 View

Can we use egg adapted influenza virus for serum neutralization test in MDCK cells ?

can we use egg adapted influenza virus for serum neutralization test in MDCK cells?Thanks

30 October 2021 2,407 4 View

Do we need adapt influenza viruses in MDCK cells before conducting serum neutralization test with influenza viruses in MDCK cells ?

Do we need adapt influenza viruses in MDCK cells before conducting serum neutralization test with influenza viruses in MDCK cels?? Thanks

30 October 2021 5,989 3 View

Anybody can give detailed protocol for VNT(virus neutralization) assay for influenza virus?

including, need of virus paasage in MDCK? dilutuion of serum 1:2 or 1:5 ? etc

12 July 2021 6,953 3 View

Gel purification of linearized plasmid, gave low yield why?

I am using Qiagen Gel extraction kit for gel purification of linearized plasmid. The procedure is followed as per the kit. if i load 1ug DNA in agarose gel, i am getting 600ng DNA or less after...

17 October 2020 6,751 4 View

Dear researchers, In RE digestion with Nco I i am getting smearing. Can any one please advice?

well1- 2 log ladder well2- uncut plasmid(3.2kb) well3- cut wit NcoI well4-cut wit NcoI tried with less time of incubation, low amount of enzyme doesnot work

11 September 2020 6,784 3 View