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Questions related from Sivasankar Panickan
how we can gel extract 5.5 kb linear plasmid? use of qiagen kit gives low yield. why?
24 May 2025 9,983 1 View
Can anyone share, primer sequence for PCR to confirm pFAST Bac Dual plasmid inserted in DH5alpha cells? probably for polyhedrin promoter
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During standard curve preparation thresold line value is 560. It should be kept same during running test samples for detecting viral rna copies?
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can we use egg adapted influenza virus for serum neutralization test in MDCK cells?Thanks
30 October 2021 2,473 4 View
Do we need adapt influenza viruses in MDCK cells before conducting serum neutralization test with influenza viruses in MDCK cels?? Thanks
30 October 2021 6,050 3 View
including, need of virus paasage in MDCK? dilutuion of serum 1:2 or 1:5 ? etc
12 July 2021 7,029 3 View
I am using Qiagen Gel extraction kit for gel purification of linearized plasmid. The procedure is followed as per the kit. if i load 1ug DNA in agarose gel, i am getting 600ng DNA or less after...
17 October 2020 6,839 4 View
well1- 2 log ladder well2- uncut plasmid(3.2kb) well3- cut wit NcoI well4-cut wit NcoI tried with less time of incubation, low amount of enzyme doesnot work
11 September 2020 6,867 3 View
