Thresold line value must be same in different runs in time pcr for calculating viral copies?

Sivasankar Panickan @Sivasankar-Panickan

16 August 2023 0 8K Report

During standard curve preparation thresold line value is 560. It should be kept same during running test samples for detecting viral rna copies?

Badges
Science topic

Similar topics
Thesis Research

More Sivasankar Panickan's questions See All

How to perform spatial search in higher dimensions efficiently?

I have a 6D grid of approximation order 10. This means, it has a million points: (x, y, z, Vx, Vy, Vz) = (10, 10, 10, 10, 10, 10). Now, I want to find those points of this grid that lie within a...

10 October 2022 2,003 0 View

Can we use egg adapted influenza virus for serum neutralization test in MDCK cells ?

can we use egg adapted influenza virus for serum neutralization test in MDCK cells?Thanks

29 October 2021 2,421 4 View

Do we need adapt influenza viruses in MDCK cells before conducting serum neutralization test with influenza viruses in MDCK cells ?

Do we need adapt influenza viruses in MDCK cells before conducting serum neutralization test with influenza viruses in MDCK cels?? Thanks

29 October 2021 6,004 3 View

Anybody can give detailed protocol for VNT(virus neutralization) assay for influenza virus?

including, need of virus paasage in MDCK? dilutuion of serum 1:2 or 1:5 ? etc

11 July 2021 6,986 3 View

Gel purification of linearized plasmid, gave low yield why?

I am using Qiagen Gel extraction kit for gel purification of linearized plasmid. The procedure is followed as per the kit. if i load 1ug DNA in agarose gel, i am getting 600ng DNA or less after...

16 October 2020 6,798 4 View

Dear researchers, In RE digestion with Nco I i am getting smearing. Can any one please advice?

well1- 2 log ladder well2- uncut plasmid(3.2kb) well3- cut wit NcoI well4-cut wit NcoI tried with less time of incubation, low amount of enzyme doesnot work

10 September 2020 6,822 3 View

How to find the list of journals with "Your Paper Your Way"?

Dear Professors and Researchers, Can anyone tell me how to find a list of journals with the choice of "Your Paper Your Way"? Thanks in Advance Regards, Siva

03 May 2020 2,611 5 View

Having a marine actinobacterium. Sequenced for 16S and found to have 98.58% similarity with Citricoccus yambaruensis. Can it be a new species?

Sequenced a marine actinobacterium for 16S, assembled and identified in Eztaxon. Obtained 98.58% similarity with Citricoccus yambaruensis. Can it be a new species? Can I go for whole genome...

09 July 2018 3,412 5 View

Any simple and efficient protocol to explore the diversity of PKS, NRPS and relevant gene in the mangrove sediments?

I would like to explore the PKS, NRPS and relevant gene diversity in the metagenomic actinobacterial DNA of the mangrove sediment samples to understand the secondary metabolite production pattern....

16 November 2017 1,751 1 View

Can we use Soxhlet to extract metabolites from the cell mass of Actinobacteria?

Can we use Soxhlet for extracting the secondary metabolites from the Actinobacteria fermented broth? Soxhlet is generally being used for dry/solid materials. If we adopt this to actinobacteria...

22 October 2017 4,262 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View