I see a clump on my Inserts(Transwell assay), what is it?

Hello, I am working on a Transwell assay, and I have been trying to optimize the assay for a long time, currently, I am working on hCMEC/D3, seeded at 2x10^4 and left for 5 days (the experiment...

11 December 2019 9,074 3 View

Is it possible to analyze colistin sulfate in a pharmaceutical drug by HPLC with UV detection?

Colistin has weak chromopheres, therefore it is usually analyzed by microbilolgy methods and not by HPLC. is it possible to analyze it by HPLC with PDA detector.

31 December 2017 717 0 View

Why PDA detectors can not withstand colored mobile phases in HPLC analysis?

In an HPLC method of analysis, Copper (II) sulfate is used in the mobile phase which makes the mobile phase blue. However this affects adversely the PDA detector where its intensity is...

08 September 2017 315 2 View

Is there a difference in the retention time of chlorhexidine acetate, and chlorhexidine hydrochloride in HPLC analysis?

In a method of analysis of chlorhexidine hydrochloride by HPLC, chlorhexidine acetate is used in system suitability. should be there a difference in retention time between chlorhexidine...

03 April 2017 1,441 5 View

How to separate and analyze simultaneously valproic acid and sodium valproate by HPLC?

Valproic acid and sodium valproate when dissolved in the mobile phase and injected into HPLC, they coelute and can not be separated as they become identical in solution. Is there a method for...

02 March 2017 3,739 5 View

What is the maximum concentration of sodium-1decanesulfonate ion pairing regent in reversed phase mobile phase?

02 March 2017 3,341 3 View

What is the function of tetrabutylammonium hydroxide in the separation of isomers of an analyte?

02 March 2017 4,101 5 View

What is the cause of bubbles formed in the mobile phase of trifluroacetic acid solution and acetonitrile mixture mobile phase in HPLC?

01 February 2017 800 9 View

How to get high resolution in cation exchange Liquid chromatography of two basic analytes?

23 January 2017 2,493 5 View

How to get high resolution in cation exchange Liquid chromatography of two basic analytes?

31 December 2016 7,988 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View