Valproic acid and sodium valproate when dissolved in the mobile phase and injected into HPLC, they coelute and can not be separated as they become identical in solution.
Is there a method for separation and analysis of both simultaneously by HPLC.
There is an equilibrium between the free acid and the anion in aqueous eluents, which is depending on the proton (H+) concentration (pH). A separation of both species is depending sofar on the pH. At low pH (
You will have to use a non-aequous mobile phase (pH neutral) that does not allow dissociation.
once you dissolve in h2o, seems like no difference betw. your analytes.
All valproatel Who dreamed up this project? You could use ICP to detect the sodium?
Using sodium as an indication of the levels of sodium valproate has the problem of having to make the assumption that the only source of sodium is stoichiometric with the levels of sodium valproate present. Doing this by HPLC with only organic solvent will result in little or no retention of the analyte. It may be that a valid method could be found using un suppressed ion chromatography.
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