Colistin has weak chromopheres, therefore it is usually analyzed by microbilolgy methods and not by HPLC.
is it possible to analyze it by HPLC with PDA detector.
Hello, I am working on a Transwell assay, and I have been trying to optimize the assay for a long time, currently, I am working on hCMEC/D3, seeded at 2x10^4 and left for 5 days (the experiment...
11 December 2019 9,198 3 View
Milliq water is used for HPLC with UV detection , but can it be used for LC/MS.
06 January 2018 2,694 12 View
In an HPLC method of analysis, Copper (II) sulfate is used in the mobile phase which makes the mobile phase blue. However this affects adversely the PDA detector where its intensity is...
08 September 2017 440 2 View
In some monographs of pharmacopoeias, TLC method is used for analysis/quantification of impurities. Does this method have adequate accuracy, precision, LOD, LOQ for such trace analysis of...
06 July 2017 2,452 0 View
3-aminopropanol is a degradation product of panthenol (active ingredient in many creams). it present in low concentrations, so sample of the cream would have a large amount of cream. so what is...
07 May 2017 1,996 2 View
In a method of analysis of chlorhexidine hydrochloride by HPLC, chlorhexidine acetate is used in system suitability. should be there a difference in retention time between chlorhexidine...
03 April 2017 1,577 5 View
Valproic acid and sodium valproate when dissolved in the mobile phase and injected into HPLC, they coelute and can not be separated as they become identical in solution. Is there a method for...
02 March 2017 3,969 5 View
In an analysis of impurity in pharmaceutical drug, sodium-1decanesulfonate is used as ion pairing agent at a concentration of 1 g per liter which is higher than the solubility of it in water. Is...
02 March 2017 3,450 3 View
In analysis of cefixime isomer, tetrabutylammonium hydroxide is used in the mobile phase at a concentration of 2.5%. why is it used? can we use tetramethylammonium hydroxide instead of it.?
02 March 2017 4,272 5 View
During an HPLC analysis of an analyte (reversed-phase) using a mixture of 1% trifluoroacetic acid/acetonitrile mixture, bubbles arises in the mobile phase and the baseline is not stable at...
01 February 2017 997 9 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View