I see a clump on my Inserts(Transwell assay), what is it?

Hello, I am working on a Transwell assay, and I have been trying to optimize the assay for a long time, currently, I am working on hCMEC/D3, seeded at 2x10^4 and left for 5 days (the experiment...

11 December 2019 9,074 3 View

Why PDA detectors can not withstand colored mobile phases in HPLC analysis?

In an HPLC method of analysis, Copper (II) sulfate is used in the mobile phase which makes the mobile phase blue. However this affects adversely the PDA detector where its intensity is...

08 September 2017 315 2 View

Do TLC methods for impurities in pharmaceutical analysis have sufficient accuracy, precision?

In some monographs of pharmacopoeias, TLC method is used for analysis/quantification of impurities. Does this method have adequate accuracy, precision, LOD, LOQ for such trace analysis of...

06 July 2017 2,326 0 View

Is there a difference in the retention time of chlorhexidine acetate, and chlorhexidine hydrochloride in HPLC analysis?

In a method of analysis of chlorhexidine hydrochloride by HPLC, chlorhexidine acetate is used in system suitability. should be there a difference in retention time between chlorhexidine...

03 April 2017 1,441 5 View

How to separate and analyze simultaneously valproic acid and sodium valproate by HPLC?

Valproic acid and sodium valproate when dissolved in the mobile phase and injected into HPLC, they coelute and can not be separated as they become identical in solution. Is there a method for...

02 March 2017 3,739 5 View

What is the maximum concentration of sodium-1decanesulfonate ion pairing regent in reversed phase mobile phase?

02 March 2017 3,341 3 View

What is the function of tetrabutylammonium hydroxide in the separation of isomers of an analyte?

02 March 2017 4,101 5 View

What is the cause of bubbles formed in the mobile phase of trifluroacetic acid solution and acetonitrile mixture mobile phase in HPLC?

01 February 2017 800 9 View

How to get high resolution in cation exchange Liquid chromatography of two basic analytes?

23 January 2017 2,493 5 View

How to get high resolution in cation exchange Liquid chromatography of two basic analytes?

31 December 2016 7,988 6 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View