Hi there

It should be OK, make sure is is ultrapure : 18.2 MΩ·cm

The prefilter and RO filter and both UV lamps (bacterial and photo oxidation) should eliminate most inorganic and organic background,.

Problem with buying LCMS grade water is that once the bottle is opened it should be used almost immediately which is not always possible.

Try to avoid plastic containers, they do contain loads of plasticizes

Thanks a lot for your answer Leonardo.

The name "Milli-Q" ® is a trademark of Millipore Corporation used to brand a type 1 grade of ultrapure water. The name is sometimes used in a generic way to describe a high purity water. For LC/MS applications we have a list of general criteria and characteristics which any water used should meet. More information on the topic can be found at the linked article below.

"What type of Water Should I use for HPLC, UHPLC or LC/MS Analysis?" https://hplctips.blogspot.com/2013/10/what-type-of-water-should-i-use-for.html

yes william sir is right.

MILI Q is a trade name of milipore corporation.

For LC MS, the requirement of water is,

It should be ultra pure grade having 18.2 MΩ·cm, passed through different filters for removal of an contamination of micro-organisms, minerals, etc.

A problem is often the sodium content. If you buy LC-MS grade water the glass bottle were produced out of sodium free glass. So you have no problem with time and solution of sodium from the glass wall. If you use normal solvent glas bottles you can see th rise of sodium-adducts in the LC-MS-Signal with time. The best is to use bought bottles from LC-MS grade water. You can refill them with the Mili-Q water.

It really isn't worth the trouble. Always get the purest solvents and you won't have to worry about chasing down strange polymers in your LC runs. One of the recommendations I got when setting up a proteomics lab was to stick to Honeywell LC solvents (water and acetonitrile), and I never had a problem.

Depends on how sensitive your system is !? We compared different suppliers of LC-MS grade solvent and found Biosolve solvents from Greyhound to be the that ones that generated the least background noise. But these differences were only visible in your most sensitive systems (e.g. Xevo TQ-S). If you're only doing single quadrupole analysis MilliQ water is perfectly fine .

I have used Millipore purified water for LC/MS for decades and prior to that we used it for purification of metabolites for FAB MS as it out performed any commercially available water. Millipore water was almost always the purest water available, especially if the bottled water was not used within a week of opening. As many of the analyst above point out, you should evaluate the water for your particular needs/application. In my current position, I typically perform high resolution LC/MS on partially purified extracts from plant, animal, or soil matrices. For these samples, any of the purified waters available for LC/MS (bottled or Milli-Q) would be more than adequate. In this case, I would use the cheapest available. However, if I was looking for trace impurities in a pharmaceutical, I would throroughly evaluate all solvents prior to use as an impurity in the water could be miconstrued as an impurity in the drug substance .

WOW! . Entedhar Sarhat : Are you familiar with the term plagiarism? You should be. You just copied my published article and posted it here as your own work without permission and without citing me as the author [ "What type of Water Should I use for HPLC, UHPLC or LC/MS Analysis?"; https://hplctips.blogspot.com/2013/10/what-type-of-water-should-i-use-for.html ]

If you look above, about three posts down, you will see that I already posted my own article in response to the question.

"The name "Milli-Q" ® is a trademark of Millipore Corporation used to brand a type 1 grade of ultrapure water. The name is sometimes used in a generic way to describe a high purity water. For LC/MS applications we have a list of general criteria and characteristics which any water used should meet. More information on the topic can be found at the linked article below.

"What type of Water Should I use for HPLC, UHPLC or LC/MS Analysis?" https://hplctips.blogspot.com/2013/10/what-type-of-water-should-i-use-for.html "

*Update: This was not a one-time event. It turns out that Entedhar Sarhat only posts on RG by copy/pasting work of others as their own to various questions on Researchgate, as their own plagiarized response, each time. RG shows over 30 plagiarized posts so far!

• 10/2018 UPDATE: After filing of 'Notice of Copyright Infringement', Entedhar's post (above) was deleted by Researchgate administrators for copyright infringement and plagiarism.

Mostly u can use LC-MS grade water as to avoid interferance of adduct like Na or other plastic components reacted with water. you can also use ultra pure grade 18.2 MΩ·cm water.

Also u may go for comparative study of water as a sample to check any unwanted peak.

Hello, I have observed PEG contamination after using water from sources other than LCMS grade water. PEG eluted at around Water:ACN (70:30) in Reverse phase LC-MS

The output water of Milli Q with LC Pak at the point of use is recommended to be used for LC-MS/MS application.LC Pak is having C18 reverse-phase Silica, which acts as guard and column, and suppresses if any trace level organic.