Why are so many researchers trying to isolate proteins from the tangled mess of coli inclusion bodies?

Granted, there are inclusion bodies that contain huge concentrations of small bioactive proteins that can be isolated without loss of bioactivity. But trying to isolate large proteins and...

06 July 2017 8,380 20 View

What is your experience in finding a job using headhunters?

04 May 2017 9,486 4 View

What is the value of cell-free DNA analysis of cancer patient blood samples?

Almost all of our cells are directly connected to the blood circulation. Thousands of our cells die every hour and spill their contents into the circulation to be cleared by the liver and...

09 October 2016 6,036 5 View

Are there companies selling purified chiral SW-carbon nanotubes?

01 February 2016 7,089 5 View

What is the viability of cancer cell lines spiked into normal whole blood?

Hi, I am looking at how long cancer cell lines remain alive after spiking them into normal whole blood samples. I have spiked live MCF-7 and SKBR-3 cells into whole blood and I lose >25% of the...

09 October 2015 7,706 19 View

Can you do ABO typing using formaldehyde fixed blood cells?

01 February 2015 8,017 4 View

Storing N-hydroxysuccinimide (NHS) esters without losing reactivity?

Hi, I have been using reactive NHS fluorescent reagents and they lose activity after the vial is opened and kept as powder at 4oC. Dissolving them in DMSO and freezing them at -20oC also results...

07 August 2014 7,264 8 View

Looking for US collaborators to evaluate red cell and platelet preservation solutions.

We are a small biotech company in Rockville MD that is developing non-toxic storage solutions for blood cells and platelets. We have promising preliminary data indicating that we can preserve red...

05 June 2013 6,672 1 View

Viable biotech company?

I,m sorry Robert. but tissue culture experiments do not attract attention of pharma companies like they used to a decade ago. Right now, pharma wants in vivo studies with mice or studies with...

01 January 1970 2,475 14 View

Giant cells in melanoma biopsies?

01 January 1970 6,332 9 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View