Why are so many researchers trying to isolate proteins from the tangled mess of coli inclusion bodies?

Granted, there are inclusion bodies that contain huge concentrations of small bioactive proteins that can be isolated without loss of bioactivity. But trying to isolate large proteins and...

06 July 2017 8,380 20 View

What is your experience in finding a job using headhunters?

04 May 2017 9,486 4 View

What is the value of cell-free DNA analysis of cancer patient blood samples?

Almost all of our cells are directly connected to the blood circulation. Thousands of our cells die every hour and spill their contents into the circulation to be cleared by the liver and...

09 October 2016 6,036 5 View

Are there companies selling purified chiral SW-carbon nanotubes?

01 February 2016 7,089 5 View

What is the viability of cancer cell lines spiked into normal whole blood?

Hi, I am looking at how long cancer cell lines remain alive after spiking them into normal whole blood samples. I have spiked live MCF-7 and SKBR-3 cells into whole blood and I lose >25% of the...

09 October 2015 7,706 19 View

Can you do ABO typing using formaldehyde fixed blood cells?

01 February 2015 8,017 4 View

Storing N-hydroxysuccinimide (NHS) esters without losing reactivity?

Hi, I have been using reactive NHS fluorescent reagents and they lose activity after the vial is opened and kept as powder at 4oC. Dissolving them in DMSO and freezing them at -20oC also results...

07 August 2014 7,264 8 View

Do megakaryocytes stain for cytokeratin?

Hi, I have heard anecdotal reports that some megakaryocytes stain positively with a pan-anti-CK (8, 18, 19) cocktail. Can anyone confirm this and/or direct me to publications that show...

05 June 2014 1,233 4 View

Looking for US collaborators to evaluate red cell and platelet preservation solutions.

We are a small biotech company in Rockville MD that is developing non-toxic storage solutions for blood cells and platelets. We have promising preliminary data indicating that we can preserve red...

05 June 2013 6,672 1 View

Viable biotech company?

I,m sorry Robert. but tissue culture experiments do not attract attention of pharma companies like they used to a decade ago. Right now, pharma wants in vivo studies with mice or studies with...

01 January 1970 2,475 14 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View