I want to generate a cell line expressing my gene under an inducible promoter. I chose FuW-tetO-MCS but I also need FUW-M2rtTA for the induction. So do I transfect both plasmids together in HEK293T and use the particles to transduce my cell line or do I transfect the two plasmids separately to obtain two sets of viral particles and then mix them for the transduction?

Thank you for your help. I am new in this field.

Najma