Do I need to transfect FuW-tetO-MCS and FUW-M2rtTA plasmids together in HEK293T or separately?

I want to generate a cell line expressing my gene under an inducible promoter. I chose FuW-tetO-MCS but I also need FUW-M2rtTA for the induction. So do I transfect both plasmids together in...

12 January 2021 8,501 2 View

Is there any thin, flexible conducting wires capable of current carrying of around 0.5-1.0 Ampere?

I want to use connecting wires for the soft robot to deliver electric current of around 1 A (voltage 5V), however currently available wires are quite rigid and they exert negative force for the...

05 January 2021 4,485 3 View

A three electrode sensor inkjet printed gets damaged in few hours of usage in a dynamic media flow chip, any solution for that?

We are using some biosensors which are inkjet printed and are used in the microfluidic chip where media is flowing at the flow rate of around 60 uL/min. However, after the continuous use of few...

05 January 2021 610 2 View

USB based DAQ options with atleast 4 analog output channels and 8 analog input channels other than National Instruments?

National Instruments provides vast variety of DAQ options which are based on LabVIEW, however they are way too expensive when it comes to opting option with high analog output channels (like 4)....

30 August 2020 5,195 6 View

How to avoid fluorescence quenching on gold nanoparticles?

I have tried several fluorophores with different excitation and emission range, from 500 to 800 nm with no luck in obtaining any positive/good fluoroscence intensity. I know that gold...

15 July 2020 3,669 0 View

What could be direct/indirect roles of insects (entomology) in current pandemic COVID-19?

Because there is no any vaccine for COVID-19 until and in past insects have transmitted number of diseases like; chikungunya virus, yellow fever, dengue fever,Lyme disease, plague,malaria,...

24 June 2020 9,557 30 View

Automatic Save STARCCM+: Warning ''Insufficient space on the disk''?

Hi Everyone, While running the simulation, I kept the file on ''Autosave'' so that each time step is saved (to avoid loss of data due to internet disruption since I am using VPN) but after 1-2...

13 May 2020 7,645 3 View

Which is better for polymer grafting, adding polymer into your nanoparticles or the other way around?

I am having problem with coating my NP (size ~100 nm, irregular shape/cluster, stabilised with citrate). So now I am trying both methods with different results, but I just find it really time...

12 February 2020 2,075 3 View

Where to purchase flexible connecting wires for soft actuators?

Normal wires (for providing voltage) to soft actuators behave as an obstacle to the actuators and hence the real response of designed soft actuators is not known. can you please share the online...

13 November 2019 5,602 1 View

How to classify a text in to different categories without predetermined labeling?

I want to classify a text of more than 1000 words into a categories. I have a database of 10,000 reports of which each contain more than 1000 words. I want to classify them and would like to know...

04 October 2019 1,554 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View