Do I need to transfect FuW-tetO-MCS and FUW-M2rtTA plasmids together in HEK293T or separately?

I want to generate a cell line expressing my gene under an inducible promoter. I chose FuW-tetO-MCS but I also need FUW-M2rtTA for the induction. So do I transfect both plasmids together in...

12 January 2021 8,501 2 View

How to avoid fluorescence quenching on gold nanoparticles?

I have tried several fluorophores with different excitation and emission range, from 500 to 800 nm with no luck in obtaining any positive/good fluoroscence intensity. I know that gold...

15 July 2020 3,669 0 View

Which is better for polymer grafting, adding polymer into your nanoparticles or the other way around?

I am having problem with coating my NP (size ~100 nm, irregular shape/cluster, stabilised with citrate). So now I am trying both methods with different results, but I just find it really time...

12 February 2020 2,075 3 View

Can anyone help me find a free full version software for data envelopment analysis?

Want to analyse PMN data.

15 February 2014 5,092 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

How to apply a voltage sinusoidal/harmonic load in ANSYS APDL?

Hello all, I want to simulate a Dielectric Elastomer Actuator DAE (an electrostatic-Structural analysis). I want to apply various voltages and frequencies into the DEA in the form of sine wave....

13 February 2021 3,261 3 View

Silane solution and application to Glass ?

30 January 2021 7,924 3 View

Forced Vibration Testing using FDD?

Hi, Can we use FDD algorithm for forced vibration response (sinusoidal response produced from eccentric mass vibrator)? Thanks

22 January 2021 5,851 2 View

What percentage torque ripple exists in electrical motors used in electric vehicles ?

I am trying to understand the significance/percentage of torque ripples that exist in electrical motors (under operating conditions) that are used in commercial electric vehicles. If someone could...

15 January 2021 5,691 3 View

Can we control the position and vibration, simultaneously, of a flexible link with a tip mass using only the actuator's input?

This is a boundary control problem of a hybrid ODE-PDE system. The system consists of an actuator, a flexible link fixed to the actuator, and a tip mass at the free end of the link. In most...

13 January 2021 8,050 2 View

Metabolomics Data Normalisation?

Hello, Setting: We have a rather large dataset consisting of somewhat 500 samples containing metabolomics data. The data was obtained by analysing dry capillary blood using HPLC methods, yielding...

04 January 2021 8,773 5 View

Does capillary blood appear more or less viscous the higher the glucose level?

Hi there! I perform a lot of blood sugars and I always wonder if the viscosity of the blood correlates to the blood sugar level. Have you had this thought and if so, what is your experience? So...

04 January 2021 832 3 View