Do I need to transfect FuW-tetO-MCS and FUW-M2rtTA plasmids together in HEK293T or separately?

I want to generate a cell line expressing my gene under an inducible promoter. I chose FuW-tetO-MCS but I also need FUW-M2rtTA for the induction. So do I transfect both plasmids together in...

13 January 2021 8,630 2 View