Hello,
I am doing a drug release analysis, placing sample in a dialysis membrane, placing membrane in a flask with water and removing water samples for analysis via hplc.
The method that I have found in jounral articles is via method 1 below.
Method 1. Sink maintained: e.g. remove 1mL sample for analysis, replace with 1mL
Method 2. Complete buffer replacement: e.g. remove 1mL sample for analysis, discard buffer and replace with fresh buffer completely.
I am using Paclitaxel which is hydrophobic and has very low solubility in water, so it makes sense (to me) to do the second way so the buffer doesnt get overconcentrated...
I am wondering if there is any reason why I shouldnt do method 2?
Kind regards,
Lara.
I don't see any reason not to do method 2. You just have to calculate the total amount of paclitaxel that was released by multiplying the concentration in the bath by the volume of the bath at each stage of the process..
I would be concerned about whether the paclitaxel will be able to get into the aqueous phase at all, considering its poor solubility. At best, this will make dialysis a very slow process, and therefore the rate of release may be underestimated.
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