15 Questions 10 Answers 0 Followers
Questions related from Theatin van Leeuwen
I have porous PMMA microparticles up to 1mm in diameter. I would like to functionalise the surface with amines whilst keeping the particles intact. Chat GTP has recommended ethanolamine or ATPES...
14 June 2024 8,084 1 View
Hi, Just wondering if anyone has found out the concentration of cysteine in the colon (or from faeces)? I have found that the amount of cysteine in blood plasma is 240-360uM (Wang, 2019) so i...
24 January 2023 4,627 1 View
Hello, I have PMMA polymer particles embedded with CuO nanoparticles. I analysed two samples via ICP-MS and obtained the Cu wt% within the expected range. I then analysed further samples via...
07 September 2022 8,836 3 View
Hello, I have done a bit of research to see if there are any cells floating about in the colon (i.e. the lumen part) and cannot find anything that suggests there is, and would like to check if...
28 July 2022 3,095 1 View
I am measuring the absorbance of a Methylene Blue gradient on a plate reader. The highest concentrations are "super dark blue" in colour and on the calibration plot begin to plateau (no longer...
24 November 2021 3,062 2 View
Hello, I would like to know the mechanism behind why "membrane impermeable dyes" are not able to penetrate through the phospholipid cell membrane. Also, does this mean they are also impermeable...
29 October 2020 3,142 3 View
Hello, Wondering if there are any physicists that can explain (very simply as I am a chem eng) whether a charged particle would move faster or slower through water than an uncharged particle....
21 October 2020 4,054 7 View
Hello, I am trying to form a pellet of PMMA particles. I have tried centrifuging for 1 hour at 26000 RPM (highest the centrifuge I have will go) but the particles do not form a pellet they...
10 September 2020 8,997 5 View
Hello, Cetyl alcohol is soluble in solvents like acetone and alcohols but not water. Cetyl alcohol is a waxy substance at room temp with a melting temp at around 50 dC. If I want to dissolve...
17 August 2020 8,277 3 View
Hello, We are trying to stain actin and tubulin with BACMAM. We have tried different concentrations and incubation times, however each time we get the same type of image. The cell is stained...
13 August 2020 1,512 1 View
Hello, I have used a 14kDa dialysis bag made from cellulose. I put some food dye in there. (10mL food dyed water and 200mL external buffer). It has taken about 3 days for equilibrium to be...
26 June 2020 2,843 2 View
I am quantifying paclitaxel on HPLC. I dissolved using two different methods. 1. Pax, SDS, 50:50 water:acetonitrile (no sonication as looked clear) 2. Pax, SDS, 100% acentonitrile (sonicated 1...
06 May 2020 688 0 View
Hello, Just wondering if anyone knows what this mixture would look like when stable and when mixed? I.e. how do the molecules distribute. Can assume that SDS is below the CMC and water and...
28 April 2020 5,763 3 View
Hello, I am doing a drug release analysis, placing sample in a dialysis membrane, placing membrane in a flask with water and removing water samples for analysis via hplc. The method that I...
09 March 2020 8,290 1 View
Hello, I just changed to a new HPLC column and running the same procedure as on the previous column my peak showed splitting and multiple peaks appeared over the run where previously there was...
12 February 2020 9,266 6 View
