I will be administering 5-fluorouracil at a dosage of 50 mg/kg to mice as an I/P injection. Will someone please check my calculation? (Mw: 130.078 g/mol)

Max volume to be administered to mice I/P = 10 ml/kg

I will be injecting 12 mice, hence the 4 mL at step 2.

1. Make up 5-FU stock solution:

I want: 1 mL of a 200 mM solution

m=M x Mr

m= 0.2 M x 130.078 g/mol

m= 26.0156 mg 5-FU in 1 mL DMSO

2. Make up 5-FU working solution in PBS:

C1V1=C2V2

(26.0156 mg/ml) (V1) = (6 mg/mL)(4 mL)

V1 = 923 uL of 1. in 3077 uL PBS

3. Injection volume

5-FU Dosage (mg/kg) x Mouse weight (kg) / treatment concentration (mg/mL)

e.g using a 20 g mouse

(50 mg/ml) x (0.02 kg) / (6 mg/ml)

= 0.167 mL

= 167 uL of 2. injected I/P into mouse

Thank you :)