I would like to work in cardiac cells. Right now am working with H9C2 cells. Can anyone help me to carry out my experiment in primary cell lines? Looking for a procedure to isolate and do passaging of those cells.
I guess by mentioning cardiac cells you meant cardiac myocytes. To begin with I would suggest you to go through the attached protocol for isolation/establishment of rat cardiomyocytes in primary culture and passaging the cells.