Which characteristic marker of H9C2 cell line can be used in lab with help of PCR?

03 April 2015 3,893 2 View

Do anyone can help me regarding primary cell lines?

I would like to work in cardiac cells. Right now am working with H9C2 cells. Can anyone help me to carry out my experiment in primary cell lines? Looking for a procedure to isolate and do...

01 February 2014 3,259 2 View

How to get rid of water added for the preparation of a protein suspension?

I am working in insulin. I have an insulin suspension. I want to remove water from that such that it can be further characterized. Thank you.

31 December 2013 7,943 7 View

Does anyone have detailed protocol for LDH cytotoxicity assay in both suspension and adherent cells?

Kindly help me with the procedure to do LDH assay.. I need to do this as this is my preliminary work

05 June 2013 4,555 0 View

How to calculate binding efficiency between protein and any other molecule?

By using SDS-PAGE we can find the proteins of various mol weight or size, but can we find whether our protein binds to any other molecule and how efficiently it binds? Also does anyone know any...

11 December 2012 3,776 8 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View