Shakila Mahalakshmi

6 Questions 13 Answers 0 Followers

Questions related from Shakila Mahalakshmi

Which characteristic marker of H9C2 cell line can be used in lab with help of PCR?

I am working with Cardiac cell line H9C2. I want to confirm whether it is exhibiting the cardiac muscle properties. Kindly tell me any marker that can be used with the help of PCR not with...

04 April 2015 3,946 2 View

Do anyone can help me regarding primary cell lines?

I would like to work in cardiac cells. Right now am working with H9C2 cells. Can anyone help me to carry out my experiment in primary cell lines? Looking for a procedure to isolate and do...

02 February 2014 3,313 2 View

How to get rid of water added for the preparation of a protein suspension?

I am working in insulin. I have an insulin suspension. I want to remove water from that such that it can be further characterized. Thank you.

01 January 2014 8,004 7 View

Does anyone have detailed protocol for LDH cytotoxicity assay in both suspension and adherent cells?

Kindly help me with the procedure to do LDH assay.. I need to do this as this is my preliminary work

06 June 2013 4,615 0 View

How to calculate binding efficiency between protein and any other molecule?

By using SDS-PAGE we can find the proteins of various mol weight or size, but can we find whether our protein binds to any other molecule and how efficiently it binds? Also does anyone know any...

12 December 2012 3,833 8 View

Can anyone recommend a method for extraction of phytochemicals in plants?

I am working in the field of phytochemistry and I have to separate the main chemicals present in plants.

04 April 2012 2,417 5 View