Do anyone can help me regarding primary cell lines?

I would like to work in cardiac cells. Right now am working with H9C2 cells. Can anyone help me to carry out my experiment in primary cell lines? Looking for a procedure to isolate and do...

01 February 2014 3,259 2 View

How to get rid of water added for the preparation of a protein suspension?

I am working in insulin. I have an insulin suspension. I want to remove water from that such that it can be further characterized. Thank you.

31 December 2013 7,943 7 View

Does anyone have detailed protocol for LDH cytotoxicity assay in both suspension and adherent cells?

Kindly help me with the procedure to do LDH assay.. I need to do this as this is my preliminary work

05 June 2013 4,555 0 View

How to calculate binding efficiency between protein and any other molecule?

By using SDS-PAGE we can find the proteins of various mol weight or size, but can we find whether our protein binds to any other molecule and how efficiently it binds? Also does anyone know any...

11 December 2012 3,776 8 View

Can anyone recommend a method for extraction of phytochemicals in plants?

I am working in the field of phytochemistry and I have to separate the main chemicals present in plants.

03 April 2012 2,356 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View