Kindly help me with the procedure to do LDH assay.. I need to do this as this is my preliminary work
I am working with Cardiac cell line H9C2. I want to confirm whether it is exhibiting the cardiac muscle properties. Kindly tell me any marker that can be used with the help of PCR not with...
03 April 2015 3,964 2 View
I would like to work in cardiac cells. Right now am working with H9C2 cells. Can anyone help me to carry out my experiment in primary cell lines? Looking for a procedure to isolate and do...
01 February 2014 3,349 2 View
I am working in insulin. I have an insulin suspension. I want to remove water from that such that it can be further characterized. Thank you.
31 December 2013 8,038 7 View
By using SDS-PAGE we can find the proteins of various mol weight or size, but can we find whether our protein binds to any other molecule and how efficiently it binds? Also does anyone know any...
11 December 2012 3,886 8 View
I am working in the field of phytochemistry and I have to separate the main chemicals present in plants.
03 April 2012 2,438 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.
06 August 2024 6,796 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View