Why are marine bacteria and their enzyme activity superior than the others ?

08 September 2014 4,180 7 View

How to achieve final voltage during IEF of serum sample?

Whenever I did 2D analysis of my serum sample, it takes too much time to reach its final voltage and also face fluctuation in voltage during IEF. Please suggest the protocol for IEF program and...

31 December 2013 1,092 4 View

How do I get final voltage in 2D analysis of serum sample?

Whenever I do 2D for serum sample, it takes a long time and the final voltage could not be achieved and the fluctuation in voltage during IEF is also a major problem with my serum sample. Please...

31 December 2013 6,156 0 View

Can anybody suggest me the protocol for protein isolation from human bones?

I am having difficulties in protein isolation.

31 December 2013 9,982 0 View

Will CHAPS solubalization of membrane protein help for the study of their interaction in cell culture?

We are not getting the comparative result for protein action in cell culture

08 September 2013 2,478 1 View

With PAGE analysis, what should ideally be performed to denature the Protein i.e. Boil or Heat?

I used both like heating sample with sample buffer at 95 to 99'C for 5 to 7 min or kept for 5 min on boiling water. The purpose of this is the same but which is the correct method?

06 July 2013 436 6 View

Can we use Triton X 100 to remove lipids from membrane protein in case of E. Coli. ?

Extraction of Membrane protein form EAEC requires to remove all the lipid contents. What should I can use for this purpose.

05 June 2013 614 4 View

How can we check that our fungal culture is mutated when we allow a fungi (trichoderma) for chemical mutation ?

i have done the mutation process for my trichoderma culture. Now i have to check the mutated fungi . what type of method we can used to check the mutation other then DNA sequencing.

31 December 2011 6,216 8 View

What is the advantage of using chromogenic medium for the identification of specific microorganism while we have the selective media for a particular organism ?

Because chromogenic medium are very costly . various company are manufacturing a list of chromogenic media for their respective organisms. but we can also apply selective media for the detection...

31 December 2011 6,478 15 View

technological Options for Sustainable Solid Waste Water Management jst give your idea from any stream microbiology biotechnology

We need a new idea to come it forword in the market and also in society

11 December 2011 8,227 0 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View