Shashank Dadsena

12 Questions 15 Answers 0 Followers

Questions related from Shashank Dadsena

Why are marine bacteria and their enzyme activity superior than the others ?

marine environment has some miracle for their metabolism and enzyme activity...

09 September 2014 4,253 7 View

Do antibiotics interfere with the process of glycosylation?

We are using antibiotics in our cell culture media. I want to check the glycosylation pattern in eukaryotic cells.

02 February 2014 3,818 3 View

How do I get final voltage in 2D analysis of serum sample?

Whenever I do 2D for serum sample, it takes a long time and the final voltage could not be achieved and the fluctuation in voltage during IEF is also a major problem with my serum sample. Please...

01 January 2014 6,233 0 View

How to achieve final voltage during IEF of serum sample?

Whenever I did 2D analysis of my serum sample, it takes too much time to reach its final voltage and also face fluctuation in voltage during IEF. Please suggest the protocol for IEF program and...

01 January 2014 1,156 4 View

Can anybody suggest me the protocol for protein isolation from human bones?

I am having difficulties in protein isolation.

01 January 2014 10,050 0 View

Will CHAPS solubalization of membrane protein help for the study of their interaction in cell culture?

We are not getting the comparative result for protein action in cell culture

09 September 2013 2,551 1 View

With PAGE analysis, what should ideally be performed to denature the Protein i.e. Boil or Heat?

I used both like heating sample with sample buffer at 95 to 99'C for 5 to 7 min or kept for 5 min on boiling water. The purpose of this is the same but which is the correct method?

07 July 2013 495 6 View

Can we use Triton X 100 to remove lipids from membrane protein in case of E. Coli. ?

Extraction of Membrane protein form EAEC requires to remove all the lipid contents. What should I can use for this purpose.

06 June 2013 679 4 View

How can we check that our fungal culture is mutated when we allow a fungi (trichoderma) for chemical mutation ?

i have done the mutation process for my trichoderma culture. Now i have to check the mutated fungi . what type of method we can used to check the mutation other then DNA sequencing.

01 January 2012 6,296 8 View

What is the advantage of using chromogenic medium for the identification of specific microorganism while we have the selective media for a particular organism ?

Because chromogenic medium are very costly . various company are manufacturing a list of chromogenic media for their respective organisms. but we can also apply selective media for the detection...

01 January 2012 6,542 15 View

How a strain of microorganism can developed a new metabolism so that they can withstand with drug .

A normal strain of microorganism suscepible for a drug, then it produses some protein which helps them for resistance,so how this change occur in molecular level

12 December 2011 851 10 View

technological Options for Sustainable Solid Waste Water Management jst give your idea from any stream microbiology biotechnology

We need a new idea to come it forword in the market and also in society

12 December 2011 8,316 0 View