Do antibiotics interfere with the process of glycosylation?

We are using antibiotics in our cell culture media. I want to check the glycosylation pattern in eukaryotic cells.

01 February 2014 3,753 3 View

How to achieve final voltage during IEF of serum sample?

Whenever I did 2D analysis of my serum sample, it takes too much time to reach its final voltage and also face fluctuation in voltage during IEF. Please suggest the protocol for IEF program and...

31 December 2013 1,092 4 View

How do I get final voltage in 2D analysis of serum sample?

Whenever I do 2D for serum sample, it takes a long time and the final voltage could not be achieved and the fluctuation in voltage during IEF is also a major problem with my serum sample. Please...

31 December 2013 6,156 0 View

Can anybody suggest me the protocol for protein isolation from human bones?

I am having difficulties in protein isolation.

31 December 2013 9,982 0 View

Will CHAPS solubalization of membrane protein help for the study of their interaction in cell culture?

We are not getting the comparative result for protein action in cell culture

08 September 2013 2,478 1 View

With PAGE analysis, what should ideally be performed to denature the Protein i.e. Boil or Heat?

I used both like heating sample with sample buffer at 95 to 99'C for 5 to 7 min or kept for 5 min on boiling water. The purpose of this is the same but which is the correct method?

06 July 2013 436 6 View

Can we use Triton X 100 to remove lipids from membrane protein in case of E. Coli. ?

Extraction of Membrane protein form EAEC requires to remove all the lipid contents. What should I can use for this purpose.

05 June 2013 614 4 View

How can we check that our fungal culture is mutated when we allow a fungi (trichoderma) for chemical mutation ?

i have done the mutation process for my trichoderma culture. Now i have to check the mutated fungi . what type of method we can used to check the mutation other then DNA sequencing.

31 December 2011 6,216 8 View

What is the advantage of using chromogenic medium for the identification of specific microorganism while we have the selective media for a particular organism ?

Because chromogenic medium are very costly . various company are manufacturing a list of chromogenic media for their respective organisms. but we can also apply selective media for the detection...

31 December 2011 6,478 15 View

technological Options for Sustainable Solid Waste Water Management jst give your idea from any stream microbiology biotechnology

We need a new idea to come it forword in the market and also in society

11 December 2011 8,227 0 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View