Why are marine bacteria and their enzyme activity superior than the others ?

08 September 2014 4,180 7 View

Do antibiotics interfere with the process of glycosylation?

We are using antibiotics in our cell culture media. I want to check the glycosylation pattern in eukaryotic cells.

01 February 2014 3,753 3 View

How to achieve final voltage during IEF of serum sample?

Whenever I did 2D analysis of my serum sample, it takes too much time to reach its final voltage and also face fluctuation in voltage during IEF. Please suggest the protocol for IEF program and...

31 December 2013 1,092 4 View

Can anybody suggest me the protocol for protein isolation from human bones?

I am having difficulties in protein isolation.

31 December 2013 9,982 0 View

Will CHAPS solubalization of membrane protein help for the study of their interaction in cell culture?

We are not getting the comparative result for protein action in cell culture

08 September 2013 2,478 1 View

With PAGE analysis, what should ideally be performed to denature the Protein i.e. Boil or Heat?

I used both like heating sample with sample buffer at 95 to 99'C for 5 to 7 min or kept for 5 min on boiling water. The purpose of this is the same but which is the correct method?

06 July 2013 436 6 View

Can we use Triton X 100 to remove lipids from membrane protein in case of E. Coli. ?

Extraction of Membrane protein form EAEC requires to remove all the lipid contents. What should I can use for this purpose.

05 June 2013 614 4 View

How can we check that our fungal culture is mutated when we allow a fungi (trichoderma) for chemical mutation ?

i have done the mutation process for my trichoderma culture. Now i have to check the mutated fungi . what type of method we can used to check the mutation other then DNA sequencing.

31 December 2011 6,216 8 View

What is the advantage of using chromogenic medium for the identification of specific microorganism while we have the selective media for a particular organism ?

Because chromogenic medium are very costly . various company are manufacturing a list of chromogenic media for their respective organisms. but we can also apply selective media for the detection...

31 December 2011 6,478 15 View

technological Options for Sustainable Solid Waste Water Management jst give your idea from any stream microbiology biotechnology

We need a new idea to come it forword in the market and also in society

11 December 2011 8,227 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View