I am having difficulties in protein isolation.
marine environment has some miracle for their metabolism and enzyme activity...
08 September 2014 4,286 7 View
We are using antibiotics in our cell culture media. I want to check the glycosylation pattern in eukaryotic cells.
01 February 2014 3,842 3 View
Whenever I did 2D analysis of my serum sample, it takes too much time to reach its final voltage and also face fluctuation in voltage during IEF. Please suggest the protocol for IEF program and...
31 December 2013 1,183 4 View
Whenever I do 2D for serum sample, it takes a long time and the final voltage could not be achieved and the fluctuation in voltage during IEF is also a major problem with my serum sample. Please...
31 December 2013 6,273 0 View
We are not getting the comparative result for protein action in cell culture
08 September 2013 2,576 1 View
I used both like heating sample with sample buffer at 95 to 99'C for 5 to 7 min or kept for 5 min on boiling water. The purpose of this is the same but which is the correct method?
06 July 2013 518 6 View
Extraction of Membrane protein form EAEC requires to remove all the lipid contents. What should I can use for this purpose.
05 June 2013 706 4 View
Because chromogenic medium are very costly . various company are manufacturing a list of chromogenic media for their respective organisms. but we can also apply selective media for the detection...
31 December 2011 6,576 15 View
i have done the mutation process for my trichoderma culture. Now i have to check the mutated fungi . what type of method we can used to check the mutation other then DNA sequencing.
31 December 2011 6,328 8 View
We need a new idea to come it forword in the market and also in society
11 December 2011 8,358 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
