Dear Kamesh,

You can store the DNA in a variety of solution including water, TE buffer, 10-20mM Tris pH 6.5-8.5 , at 4C, -20C and -80C and additional options.

Be aware that it is not less important to make sure that the concentration of the DNA is not too low as it may get adsorbed/stick to the test tube and get excluded from your solution. I would recommend at least 70ng/ul.

DNA can be stored at -80C, but it would be more stable in Tris-EDTA than water.

It is not necessary to store DNA in -80, and it can be stored in the mentioned buffer in +4 even for long time.

Agreed

you are probably better storing at -20C

1mM EDTA solution is good enough to store DNA for years at -80*C. .

Dear Kamesh,

You can store the DNA in a variety of solution including water, TE buffer, 10-20mM Tris pH 6.5-8.5 , at 4C, -20C and -80C and additional options.

Be aware that it is not less important to make sure that the concentration of the DNA is not too low as it may get adsorbed/stick to the test tube and get excluded from your solution. I would recommend at least 70ng/ul.

Ghil makes a very good point. In addition, note that if DNA is in pure water, it will be singlestranded. So depending what you might eventually use it for, it may be better stored in TE. And unless "snap" frozen, the DNA may well be concentrated at the bottom of the tube and requre thorough mixing before samples are taken.

At room temperature high molecular DNA is in its native form even in water, ends of linear molecules may be partially melted. Yes, for a long time better to store concentrated DNA solutions(more than 100 microgram/ml) Because some DNases are active in presence Mg++ (Mn++) and Ca++ better to add to water some (1-5 mM) EDTA. To prevent DNA apurinization, pH of DNA solutions has to be above 5.0.

0 solution

See "Denaturation of DNA" Thomas R, Gene 135, (1993) 77-79 in which he states that hyperchromicity of DNA ocurrs at 20oC if the salt concentrations are sufficiently low.