my 100 bp and 1kb ladder are not separating properly in gel inspite of providing the optimum agarose concentration, voltage and running time. the individual size bands dont spatially separate properly and appear clumped. what is the solution?
If i want to test a particular compound/metabolite on my yeast strains ( which have been transformed with the desired disease plasmid and shows the phenotype) . Then what is the method that I...
06 July 2018 3,182 0 View
In plant pathology experiments generally disease severity measured in terms of grades of affected leaf area in relation to time is used for plotting the area under the disease progress...
05 June 2018 8,058 0 View
Which is a better one to suspend extracted DNA? TE buffer or ultrapure water?
05 June 2018 3,942 4 View
Can a leaf tissue be suspended in CTAB buffer until its DNA extraction? like for example if i go to a field and collect the leaf tissue and suspend the leaf tissues in CTAB in an eppendorf tube...
05 June 2018 1,717 1 View
IS CTAB buffer method of DNA extraction suitable for extracting insect DNA?
05 June 2018 8,074 2 View
In what way does preheating of CTAB buffer prior to DNA extraction from leaf tissues improve the efficacy of DNA extraction or DNA recovery
05 June 2018 3,705 4 View
I have cloned my virus in puc 18 vector. Right now Iam maintaining the clones by repeated subculture on lb medium amended with ampicillin. Is it possible to maintain the clones in glycerol...
04 May 2018 3,604 2 View
plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?
03 April 2018 1,618 3 View
Is it possible to make a readymix of PCR reaction cocktail involving components like water, dntps, taq polymerase etc. and keep it under long term storage for future use or should we use the mix...
03 April 2018 9,933 3 View
Can we store DNA (diluted with sterile distilled water of milliq water) at minus 80 degree celsius ?
02 March 2018 10,100 9 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...
31 July 2024 3,333 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View