my 100 bp and 1kb ladder are not separating properly in gel inspite of providing the optimum agarose concentration, voltage and running time. the individual size bands dont spatially separate properly and appear clumped. what is the solution?
If i want to test a particular compound/metabolite on my yeast strains ( which have been transformed with the desired disease plasmid and shows the phenotype) . Then what is the method that I...
06 July 2018 3,077 0 View
In plant pathology experiments generally disease severity measured in terms of grades of affected leaf area in relation to time is used for plotting the area under the disease progress...
05 June 2018 7,948 0 View
Which is a better one to suspend extracted DNA? TE buffer or ultrapure water?
05 June 2018 3,821 4 View
Can a leaf tissue be suspended in CTAB buffer until its DNA extraction? like for example if i go to a field and collect the leaf tissue and suspend the leaf tissues in CTAB in an eppendorf tube...
05 June 2018 1,556 1 View
IS CTAB buffer method of DNA extraction suitable for extracting insect DNA?
05 June 2018 7,925 2 View
In what way does preheating of CTAB buffer prior to DNA extraction from leaf tissues improve the efficacy of DNA extraction or DNA recovery
05 June 2018 3,579 4 View
I have cloned my virus in puc 18 vector. Right now Iam maintaining the clones by repeated subculture on lb medium amended with ampicillin. Is it possible to maintain the clones in glycerol...
04 May 2018 3,469 2 View
plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?
03 April 2018 1,479 3 View
Is it possible to make a readymix of PCR reaction cocktail involving components like water, dntps, taq polymerase etc. and keep it under long term storage for future use or should we use the mix...
03 April 2018 9,805 3 View
Can we store DNA (diluted with sterile distilled water of milliq water) at minus 80 degree celsius ?
02 March 2018 9,940 9 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
28 February 2021 8,466 2 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View