Long term storage of leaf tissue in CTAB buffer?

Kamesh Krishnamoorthy @Kamesh_Krishnamoorthy2

06 June 2018 1 2K Report

Can a leaf tissue be suspended in CTAB buffer until its DNA extraction? like for example if i go to a field and collect the leaf tissue and suspend the leaf tissues in CTAB in an eppendorf tube and store it in minus 20 degree till i proceed for DNA extraction.

Kamesh Krishnamoorthy

@miral ladani...thanks for the answer mam... actually I will be collecting the leaf samples from the field. I decide to put the plucked leaves from field directly in an eppendorf tube containing ctab. Post that I will be traveling back from field due to which the leaf suspended in CTAB may experience room temperature for around 10 hours. Iam trying to immediately suspend the leaf tissue in CTAB to avoid phenolic contamination during my DNA extraction later.is it ok mam?

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