I have cloned my virus in puc 18 vector. Right now Iam maintaining the clones by repeated subculture on lb medium amended with ampicillin. Is it possible to maintain the clones in glycerol stock..if so what is the optimum concentration of glycerol stock that will be required
Hello Kamesh,
Yes it is good an idea to preserve your clones or even infectious clones in glycerol stocks at -80C. Here I'm providing link were you can follow the protocol, we are following same steps when preparing the stocks.
https://www.addgene.org/protocols/create-glycerol-stock/.
You can use 50% of glycerol stock.
Just mix 500 ul of your overnight cloned (puc 18 vector in LB medium + Ampicillin) with 500 ul of 50% glycerol stock (Final concentration 25%). Then store at -80C for long term use.
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