Lahiru Chamara Weerasinghe Arachchige

5 Questions 26 Answers 0 Followers

Questions related from Lahiru Chamara Weerasinghe Arachchige

How to find/predict transcription factors for a putative promoter of the gene of my interest?

Dear All, Based on ORCA conserved regions, CpG islands, and histone methylation, I predict a putative promotor for my gene of interest with one of my colleague's help. I need to find/predict...

22 February 2020 3,153 3 View

Good protocol for Mouse ESC electroporation?

Dear all, Would you kindly share with me a good protocol for mouse embryonic stem cells electroporation using Neon Transfection System? What parameters and plasmid concentrations that I should...

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How to do Dendrite Spine Quantification after Rapid Golgi Staining using ImageJ ?

Hi all, I am doing Rapid Golgi staining for mice cerebellum using the FD NeuroTechnologies kit. I need to to do the dendrite spine qualifications of Purkinje cells . Does anyone know how to use...

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CMV and T7 Promoters

Hi all, What is the advantage of using the combination of CMV and T7 promoters in mammalian cells to drive the expression? Origene Rc201776 (https://www.origene.com/drawmapbysku?SKU=RC201776)...

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Which epitopes-tags are more suitable for mass spectrometer analysis, with less background?

Dear All, In order to understand the protein interactome of my gene of interest, I am planning to tag the endogenous locus of the gene with V5 epitope and to perform mass spectrometry-based...

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