How do I computationally predict the binding position of a DNA binding protein on the promoters of differentially expressed genes?

I have a list of genes that have been proven to be up-regulated in a human disease condition of interest. I also have a protein which according to literature is said to be a DNA binding protein. I...

04 May 2019 3,087 2 View

Are there any recommendable papers which elucidate best practices in the presentation of RNA-Seq data?

Please can any one suggest to me articles that highlight or explain the best practices in the presentation of bulk RNA seq data? Thank you in advance.

01 February 2019 7,412 1 View

Is there a data base, tool or method I can use to find out which of my genes code for cytokine receptors?

I have a list of differentially expressed genes from my single cell RNA seq data analyses. I want to proceed to find out which of my genes code for cytokine receptors so that I can show on a...

01 February 2019 4,443 1 View

Given a list of cell surface genes, is there a computational possibility to predict their interaction partners on the surface of another cell?

Hi folks. After performing differential expression analyses on my single cell RNA sequencing data from ILC2s of mouse origin, treated under three conditions, I generated a list of differentially...

31 December 2018 8,097 3 View

How do I identify which differentially expressed genes are cell surface genes in mice?

Hi colleagues. I am very new to single cell sequencing and IPA analyses, but I have a background in R and Python programming. In a previous study from my research group, it was identified that two...

31 December 2018 1,609 1 View

Any protocol for culture cell surface coating with PAN Biotech 2% gelatine-solution?

Dear colleagues, does someone please has an idea on the protocol that can be used to coat cell culture surfaces with 2% PAN Biotech Gelatine-Solution ? The cells I wish to culture are endothelial...

11 December 2016 4,010 0 View

Is porcine gelatin optimal for coating surfaces for culture of human endothelial colony forming cells?

05 June 2016 1,975 0 View

What is the maximum number of ECFCs (endothelial colony forming cells) that can be frozen in one vial?

04 May 2016 4,085 3 View

Can I please get literature on the co-culture of PBMCs with endothelial cells?

Hello dear colleagues, Please, I need help in finding literature about co-culturing PBMCs (Peripherial mononuclear cells) with endothelial cells(or endothelial colony forming cells)..This...

02 March 2016 4,103 2 View

What is the optimal number of freeze-thaw cycles for immortalised HUVECs?

Dear colleagues, I wish to find out, if anyone has an idea on the optimal number of freez-thaw cycles for an immortalised HUVEC cell line. I first thawed these cells from liquid nitrogen, cultured...

01 January 1970 4,710 5 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View