Anchang Charles Gwellem

11 Questions 8 Answers 0 Followers

Questions related from Anchang Charles Gwellem

How do I computationally predict the binding position of a DNA binding protein on the promoters of differentially expressed genes?

I have a list of genes that have been proven to be up-regulated in a human disease condition of interest. I also have a protein which according to literature is said to be a DNA binding protein. I...

05 May 2019 3,156 2 View

Are there any recommendable papers which elucidate best practices in the presentation of RNA-Seq data?

Please can any one suggest to me articles that highlight or explain the best practices in the presentation of bulk RNA seq data? Thank you in advance.

02 February 2019 7,470 1 View

Is there a data base, tool or method I can use to find out which of my genes code for cytokine receptors?

I have a list of differentially expressed genes from my single cell RNA seq data analyses. I want to proceed to find out which of my genes code for cytokine receptors so that I can show on a...

02 February 2019 4,502 1 View

Given a list of cell surface genes, is there a computational possibility to predict their interaction partners on the surface of another cell?

Hi folks. After performing differential expression analyses on my single cell RNA sequencing data from ILC2s of mouse origin, treated under three conditions, I generated a list of differentially...

01 January 2019 8,158 3 View

How do I identify which differentially expressed genes are cell surface genes in mice?

Hi colleagues. I am very new to single cell sequencing and IPA analyses, but I have a background in R and Python programming. In a previous study from my research group, it was identified that two...

01 January 2019 1,665 1 View

Any protocol for culture cell surface coating with PAN Biotech 2% gelatine-solution?

Dear colleagues, does someone please has an idea on the protocol that can be used to coat cell culture surfaces with 2% PAN Biotech Gelatine-Solution ? The cells I wish to culture are endothelial...

12 December 2016 4,075 0 View

DMEM or EGM-2 for culture of HUVECS?

Hello ladies and gentlemen...Can HUVECS also be optimally cultured in DMEM or EGM-2 media? Which of these two is better?

07 July 2016 4,789 2 View

Is porcine gelatin optimal for coating surfaces for culture of human endothelial colony forming cells?

Hi comrades, I wish to ask if using porcine gelatin to coat culture surfaces is optimal for culturing human ECFCs. I would also appreciate information about the optimal concentration of porcine...

06 June 2016 2,037 0 View

What is the maximum number of ECFCs (endothelial colony forming cells) that can be frozen in one vial?

Assuming the use of 90%FCS+10% DMSO as cryoprotectant and 1ml of final volume. Thank you in advance

05 May 2016 4,145 3 View

Can I please get literature on the co-culture of PBMCs with endothelial cells?

Hello dear colleagues, Please, I need help in finding literature about co-culturing PBMCs (Peripherial mononuclear cells) with endothelial cells(or endothelial colony forming cells)..This...

03 March 2016 4,188 2 View

What is the optimal number of freeze-thaw cycles for immortalised HUVECs?

Dear colleagues, I wish to find out, if anyone has an idea on the optimal number of freez-thaw cycles for an immortalised HUVEC cell line. I first thawed these cells from liquid nitrogen, cultured...

01 January 1970 4,806 5 View