How do I computationally predict the binding position of a DNA binding protein on the promoters of differentially expressed genes?

I have a list of genes that have been proven to be up-regulated in a human disease condition of interest. I also have a protein which according to literature is said to be a DNA binding protein. I...

04 May 2019 3,087 2 View

Are there any recommendable papers which elucidate best practices in the presentation of RNA-Seq data?

Please can any one suggest to me articles that highlight or explain the best practices in the presentation of bulk RNA seq data? Thank you in advance.

01 February 2019 7,412 1 View

Is there a data base, tool or method I can use to find out which of my genes code for cytokine receptors?

I have a list of differentially expressed genes from my single cell RNA seq data analyses. I want to proceed to find out which of my genes code for cytokine receptors so that I can show on a...

01 February 2019 4,443 1 View

Given a list of cell surface genes, is there a computational possibility to predict their interaction partners on the surface of another cell?

Hi folks. After performing differential expression analyses on my single cell RNA sequencing data from ILC2s of mouse origin, treated under three conditions, I generated a list of differentially...

31 December 2018 8,097 3 View

How do I identify which differentially expressed genes are cell surface genes in mice?

Hi colleagues. I am very new to single cell sequencing and IPA analyses, but I have a background in R and Python programming. In a previous study from my research group, it was identified that two...

31 December 2018 1,609 1 View

Any protocol for culture cell surface coating with PAN Biotech 2% gelatine-solution?

Dear colleagues, does someone please has an idea on the protocol that can be used to coat cell culture surfaces with 2% PAN Biotech Gelatine-Solution ? The cells I wish to culture are endothelial...

11 December 2016 4,010 0 View

DMEM or EGM-2 for culture of HUVECS?

Hello ladies and gentlemen...Can HUVECS also be optimally cultured in DMEM or EGM-2 media? Which of these two is better?

06 July 2016 4,697 2 View

Is porcine gelatin optimal for coating surfaces for culture of human endothelial colony forming cells?

05 June 2016 1,975 0 View

What is the maximum number of ECFCs (endothelial colony forming cells) that can be frozen in one vial?

04 May 2016 4,085 3 View

What is the optimal number of freeze-thaw cycles for immortalised HUVECs?

Dear colleagues, I wish to find out, if anyone has an idea on the optimal number of freez-thaw cycles for an immortalised HUVEC cell line. I first thawed these cells from liquid nitrogen, cultured...

01 January 1970 4,710 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View