How do I computationally predict the binding position of a DNA binding protein on the promoters of differentially expressed genes?

I have a list of genes that have been proven to be up-regulated in a human disease condition of interest. I also have a protein which according to literature is said to be a DNA binding protein. I...

04 May 2019 3,087 2 View

Are there any recommendable papers which elucidate best practices in the presentation of RNA-Seq data?

Please can any one suggest to me articles that highlight or explain the best practices in the presentation of bulk RNA seq data? Thank you in advance.

01 February 2019 7,412 1 View

Is there a data base, tool or method I can use to find out which of my genes code for cytokine receptors?

I have a list of differentially expressed genes from my single cell RNA seq data analyses. I want to proceed to find out which of my genes code for cytokine receptors so that I can show on a...

01 February 2019 4,443 1 View

Given a list of cell surface genes, is there a computational possibility to predict their interaction partners on the surface of another cell?

Hi folks. After performing differential expression analyses on my single cell RNA sequencing data from ILC2s of mouse origin, treated under three conditions, I generated a list of differentially...

31 December 2018 8,097 3 View

How do I identify which differentially expressed genes are cell surface genes in mice?

Hi colleagues. I am very new to single cell sequencing and IPA analyses, but I have a background in R and Python programming. In a previous study from my research group, it was identified that two...

31 December 2018 1,609 1 View

DMEM or EGM-2 for culture of HUVECS?

Hello ladies and gentlemen...Can HUVECS also be optimally cultured in DMEM or EGM-2 media? Which of these two is better?

06 July 2016 4,697 2 View

Is porcine gelatin optimal for coating surfaces for culture of human endothelial colony forming cells?

05 June 2016 1,975 0 View

What is the maximum number of ECFCs (endothelial colony forming cells) that can be frozen in one vial?

04 May 2016 4,085 3 View

Can I please get literature on the co-culture of PBMCs with endothelial cells?

Hello dear colleagues, Please, I need help in finding literature about co-culturing PBMCs (Peripherial mononuclear cells) with endothelial cells(or endothelial colony forming cells)..This...

02 March 2016 4,103 2 View

What is the optimal number of freeze-thaw cycles for immortalised HUVECs?

Dear colleagues, I wish to find out, if anyone has an idea on the optimal number of freez-thaw cycles for an immortalised HUVEC cell line. I first thawed these cells from liquid nitrogen, cultured...

01 January 1970 4,710 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

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28 February 2021 9,936 2 View