I need paper of comparison of the effects of different cryoprotectants on mesenchymal stem cells and peripheral mononuclear cells. It would be better if its about Dextran-40 and HES.
Hi
My experience and research is on PBMC/DC more than Stems, but I would caution against using starches such as Dex-40 alone to protect cells during cryo:
1. you will lose cells during dehydration/re-freezing due to intracellular and extracellular ice crystal formation. Dex-40 can help with the extra-, but you need something else
2. DMSO, while toxic, is still the go-to as it is polar, replaces water in the cell, and is minimally toxic at 5-10%
3. Think about a mix of dex-40, DMSO 10%, and Human Serum albumin to make the cells feel like they're at home. Think about preserving cells in all dimensions.
4. Trehalose sugar is useful for preventing ice crystallization
Good Luck
Bruce
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Methods
- Cloning