Omur Besbinar

I don't work with cells and your methods, but I work with the fundamental problem of quantum effects in aqueous solutions. Quantum fluctuations in water 2 nm in size interact with a 2 nm quantum dot. If its size is 4 nm, there will be no such interaction. Fluorescence in the system water + quantum dots can determine the minimum isothermal compressibility of water on its temperature dependence. A simple explanation for your problem looks like this. Quantum fluctuations appear on the kinetic properties of water. Therefore, the internationalization of cells can be seen by flow cytometry. The confocal image loses either the static nature of the method or the small size of the 3-D coverage of the object of observation. I posted the same problems for discussion

https://www.researchgate.net/post/Nuclear_quantum_effect_and_hydrogel

Thank you very much for your reply. Forgive me, as I'm a biologist, I have trouble understanding some of the physics and chemistry terminologies. So if I sonicate less and work on internalization of larger aggregates, do you think it will help to see in Confocal?

Omur Besbinar

The robot does not tell the consultant your question if you do not click on his name. See below. Try your suggestion or change the excitation frequency in the microscope if possible.