Hello, I want to grow Mycobacterium smegmatis bacteria on Lb agar and lb broth medium. Do you have a protocol on how to enlarge? Could you help?

More Hasret Araz's questions See All

Where to find samples of "answer to key selection criteria" for early carrier lectures/postdoctoral positions in Australian job market?

I currently finished my PhD studies in Australia and applying for different lecturer/post-doc positions in the same country. If you are working in the Australian market you should know we have to...

16 November 2019 5,696 3 View

What reo means in formwork context?

I was looking into formwork concrete literature and see this slide. Could you please have a look to attached slide and let me know what does reo mean? Best,

12 August 2017 4,854 3 View

Anyone know about how can I use STOMP function to plot capillary pressure vs saturation in mixed wet circular tube?

I have to come the attached plot with using STOMP function to consider different contact angles in different pore sizes for considering mixed wettability.

03 July 2017 1,319 1 View

Different Cryoprotectans on Stem Cells and Peripheral Mononuclear Cells?

I need paper of comparison of the effects of different cryoprotectants on mesenchymal stem cells and peripheral mononuclear cells. It would be better if its about Dextran-40 and HES.

12 April 2017 3,906 2 View

How to code a summation when its lower bound is a vector?

For the purpose of coding a production planning problem, I am using a programming language which is named "Julia". In the formulations, there is a formula with a lower bound which is a vector....

03 April 2017 8,380 5 View

How can I make a nonlinear expression linear?

I am trying to linearize two nonlinear functions which I attached. Please check the attachment there I clearly explained the formulations. Thank you for your comments

30 March 2017 4,036 8 View

Tumor Imaging System?

I will work on a project. Which method should I use to imaging the tumor on the mice?

09 March 2017 8,842 6 View

How can I model a production line in AIMMS with the aim of time minimization?

I want to model a production line. The objective function is to minimize production time in the production line which will be feasible via workers collaboration. Each production line have several...

20 December 2016 978 3 View

What kind of corporations or consultancy firms are engaged with multi criteria decision-making in Australia?

I am a PhD student in business school of RMIT based in Melbourne, Australia. My research topic is behavioral aspects of multi-criteria decision-making especially PROMETHEE. In fact, I am very...

07 May 2016 4,069 1 View

How PROMETHEE and Kahneman theories like prospect theory can be integrated to develop a new model? does it already worked?

I am familiar with PROMETHEE pretty much and currently get to know Kahneman ideas. I guess integration of PROMETHEE and prospect theort, for example, could be a very interesting for generate a...

09 April 2016 593 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

25 June 2024 9,151 6 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

19 June 2024 6,404 4 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

I am working on antimicrobial effect of CBD (Cannabidiol), how should I prepare the stock which dissolve in Muller Hinton agar?

I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...

10 June 2024 9,564 6 View

Which is the best media for revival of Pseudomonas aeruginosa?

Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...

09 June 2024 6,651 9 View