This virus is very fresh on the market and no articles have been published yet.
I have never worked with hSyn promoter and wondering if it as good as CAG promoter for example?
If you know any other good CRE-dependent retrograde constructs (which require only one injection in CRE-animal), i will appreciate for any advises.
Thank you in advance!
Hi Elina ! We just tried it and are skeptical about our results as the retrovirus had not been transported in our regions of interest. But we saw EGFP in other regions, more cortical so it seems to work at some point :) It just needs some improvements of the protocols I guess.
I can not help you more than that for now, good luck ! ^^
Hi, Noemie!
Thank you for your answer. Good to know taht it works somehow :) Can you conclude from your results that it has been transported retrogradely? And what about injection site? Is there any fluorescence?
Yes it has been retrogradely transported and we still see labeling in the injection site, but we had co-injected another normal tag with another color to be sure ^^
Thank you, Noemie!
We ordered it and also going to try. As a "normal tag" do you mean fluorescent dye or some Cre-dependent expression of some fluorescent protein in the cell body only (additional virus)?
Thank you in advance!
We had injected fluospheres or retrobeads ! Please let me know if it works for you :)
Best !
Hi Elina, did it work for you? would also be interested in this! Best, Chiara
Hi, Noemie Mermet-Joret and Chiara Toschi !
I apologize for the delayed answer.
Yes, retro-rAAV2-hSyn-DIO-EGFP virus worked good for us from the second trial (do not know what the reason :)). We can see nice projections, but we don't see the injection site. Thinking about combination with some other fluo-markers, probably fluospheres or retrobeads, as Noemie suggested.
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